![]() ![]() Look for flies expressing the reporter gene.Grow flies and cross to remove genetic variation between the cells of the organism (see above).The insertion tends to occur near actively transcribed genes, as this is where the chromatin structure is loosest, so the DNA most accessible. Random transposition occurs, inserting the reporter gene randomly.coli reporter gene and origin of replication, etc.). Microinject the embryo with DNA coding for transposase and a plasmid with the reporter gene and transposase recognition sequences (and often the E.Several lines of flies are required so comparison can take place and ensure that no additional genes have been knocked out. It is important to note that the inserted gene may have damaged the function of one of the host's genes. These carry the inserted gene of interest, so can be investigated to determine the phenotype due to the gene of interest. By breeding only the genotype of the gametes is passed on, removing this variation). (Only some of the cells of the organism will have been transformed. Grow flies and cross to remove genetic variation between the cells of the organism.Random transposition occurs, inserting the gene of interest and reporter gene. ![]()
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